Supporting Informations
نویسندگان
چکیده
Human prostate specific antigen (PSA) standard solution and prostate specific antibody (Ab1 and Ab2) were purchased from Shenggong Bioengineering Ltd Company (Shanghai, China). Glutaraldehyde (25% aqueous solution), bovine serum albumin (BSA), (3-aminopropyl) triethoxysilane (APTES), Tri(2,2′-bipyridyl) dichlororuthenium (II) hexahydrate, and chitosan (CS) were obtained from Sigma-Aldrich Chem. Co. (St. Louis, MO, USA). Glucose oxidase (GOD) and silica nanoparticles (99.5%, 50±5 nm) were from Aladdin (USA). Carboxylic multiwalled carbon nanotubes (MWCNTs, OD:>50 nm, length: 0.5–2 m, purity≧95%) dispersion liquid was obtained from Nanjing Xianfeng Nanomaterials Technology Co. Ltd (Nanjing, China). H2O2 (analytical reagent grade) was from Shanghai Lingfeng Chemical Reagent Co. Ltd (Shanghai, China). D-(+)-glucose and tripropylamine (TPrA) were bought from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Whatman grade 1 chromatographic paper (200.0mm×200.0mm, pure cellulose paper) was purchased from GE Healthcare Worldwide (Shanghai, China) and used with further adjustment of paper size. The ECL solution, which was comprised of Ru(bpy)3 and TPrA, were prepared in 0.1M phosphate buffer solution (PBS, pH7.4). The Millipore (model milli-Q) purified water was used in all the experiments. All the other chemicals were of analytical reagent grade without any further purification.
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